Microfluidic chip-based aptasensor for amplified electrochemical detection of human thrombin
نویسندگان
چکیده
منابع مشابه
Electrochemical aptasensor for tetracycline detection.
An electrochemical aptasensor was developed for the detection of tetracycline using ssDNA aptamer that selectively binds to tetracycline as recognition element. The aptamer was highly selective for tetracycline which distinguishes minor structural changes on other tetracycline derivatives. The biotinylated ssDNA aptamer was immobilized on a streptavidin-modified screen-printed gold electrode, a...
متن کاملVesicular aptasensor for the detection of thrombin.
Self-assembled phospholipid vesicles are functionalized with thrombin-binding aptamers using a thiol-click reaction. The resulting aptasensors signal the binding of the analyte to the vesicle surface by changes of the emission properties of membrane co-embedded reporter dyes.
متن کاملDetection of lead ions using an electrochemical aptasensor
The aim of this research was to develop a simple, selective and sensitive aptasensor for detection of Pb2+ based on hairpin structure of complementary strand (CS) of aptamer (Apt). Screen printed carbon electrode (SPCE) was electrodeposited by gold nanoparticles to be used as the substrate for the immobilization of Apt-CS. Moreover, gold nanoparticles (AuNPs) and thionine were added to increase...
متن کاملAn amplified electrochemical aptasensor for thrombin detection based on pseudobienzymic Fe3O4-Au nanocomposites and electroactive hemin/G-quadruplex as signal enhancers.
A sensitive and selective electrochemical aptasensor for thrombin detection was constructed based on hemin/G-quadruplex as the signal label and Fe3O4-Au nanocomposites with glucose oxidase (GOx-) and peroxide-mimicking enzyme activity as the signal enhancers. Due to their large surface area and good biocompatibility, Fe3O4-Au nanocomposites were employed to immobilize electroactive hemin/G-quad...
متن کاملDevelopment of an aptasensor for electrochemical detection of tetracycline
Developing the rapid, simple and sensitive biosensor system for tetracycline detection is very important in food safety. In this paper, we developed a label-free aptasensor for electrochemical detection of tetracycline. The reorganization of tetracycline binding aptamer was confirmed by Isothermal Titration Calorimetry, Kd 1⁄4 5.18 10 5 mol L 1. According to the electrochemical impendence spect...
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ژورنال
عنوان ژورنال: Electrochemistry Communications
سال: 2010
ISSN: 1388-2481
DOI: 10.1016/j.elecom.2009.12.008